人参毛状根生物合成熊果苷的研究

Studies on the Arbutin Biosynthesis by Hairy Root of Panax ginseng C.A Mayer

目的 :研究人参毛状根生物合成熊果苷的基本条件。方法 :以熊果苷的含量、氢醌 (1,4 对苯二酚 )的转化率为指标 ,找出适于生物合成的培养条件、持续时间和底物浓度。结果 :培养 2 2d的人参毛状根更换含底物的B5培养基后 ,对浓度为 2mmol·L-1的氢醌持续转化 2 4h ,所合成的熊果苷占干重的 13.0 % ,转化率达 89.0 %。结论 :本文首次以人参毛状根为反应器 ,人工合成出人参属植物所不具有的熊果苷。

Objective: To study basic conditions for biosynthesis arbutin by the hairy root of Panax ginseng . Method:Based on the content of arbutin and the biotransformation rate of hydroquione, these conditions such as the culture stage, the lasting time and the concentration of hydroquione fiting for biosynthesis were found. Result: After cultured for 22 days, transferred in fresh B 5 medium having 2 mmol·L -1 hydroquione ,the hairy root biotransformated hydroquione into arbutin in 24 hours, the bioconversion rate was 89.0% and the content of arbutin in dry root was 13.0% . Conclusion: Arbutin, was which never found in the genus of Panax ginseng , can be biosynthesised by the hairy root of Panax ginseng .