黄芪皂苷甲对组胺引起的大鼠脑软膜微血管内皮增加的影响

Effect of Satragaloside Ⅳ on the Increase of Microvascular Permeability Induced by Histamine in Pial Microvessels of Rat

目的 :观察黄芪皂苷甲对组胺引起的脑软膜微血管通透性增加的抑制作用。方法 :脑软膜微血管通透性用跨脑软膜微血管内皮的电阻表示 ,跨脑软膜微血管内皮的电阻通过将微电极插入微血管腔内记录 ,并用电缆公式计算获得。结果 :脑软膜微血管经灌流人工脑脊液 ,测得的跨内皮电阻大约为 2 5 0 0Ω·cm2 ,说明微血管内皮是具有较低通透性的致密屏障 ,在人工脑脊液中加入 10 -4 mol·L-1组胺引起跨内皮电阻快速的和可逆性的下降。在配对实验中 ,10 -4 mol·L-1组胺引起跨内皮电阻的下降能够被在人工脑脊液中加入 0 .8× 10 -4 mol·L-1黄芪皂苷甲所抑制。结论 :实验结果表明组胺能够引起脑微血管通透性增加 ,而黄芪皂苷甲能够抑制组胺引起脑微血管通透性增加。有关黄芪皂苷甲引起通透性反应的细胞机制需进一步研究。

Objective: To study the effect of satragaloside Ⅳ on the microvascular permeability induced by histamine in pial microvessels. Method: The microvascular permeability was expressed by changes in the transendothelial electrical resistance which was measured with technique using microelectrode impaled into the vascular lumen and based on cable analysis of vessels in rat. Result: The transendothelial electrical resistance of microvessels superfused with artificial cerebrospinal fluid was about 2500 Ω·cm 2, indicating a tight barrier with extremely low ion permeability, and application of 10 -4 mol·L -1 histamine in superfusate caused a rapid and reversible decrease in transendothelial electrical resistance. In paired experiment, the decrease of transendothelial electrical resistance induced by 10 -4 mol·L -1 histamine was inhibited by adding 0.8×10 -4 mol·L -1 satragaloside Ⅳ in superfusate. Conclusion: The results indicated that increases in the microvascular permeability induced by histamine, and satragaloside Ⅳ can inhibit the increases in the microvascular permeability induced by histamine. It is necessary that the cellular mechanism of permeability response induced by satragaloside Ⅳ be further elucidated.