番红花红褪色光度法测定痕量铜

Decolouring spectrophotometric determination of trace copper

利用铜 ( )对过氧化氢氧化番红花红使其褪色的催化作用 ,建立了分光光度法测定痕量铜的新方法。研究了反应条件 ,测定了反应的级数和表观活化能。该方法的线性范围为 0～ 2 4 0 ng/m L Cu2 + ,检出限为 2 .5× 1 0 -9g/m L。用该法对人发中的铜含量进行了测定 。

The decolouring oxidation of safranine by hydrogen peroxide in ammonia and ammonium chloride medium using copper as catalyst was investigated. A new method for determination of Cu based on this was set up.The linear range was 0～6 μg/25 mL with detection limit of 2.5×10 -9 g/mL for Cu.The method was used to determine trace Cu in hair samples.