植入脑源性神经营养因子载体细胞对幼鼠缺氧缺血性脑损伤后记忆的影响

Effect of transplantation of genetically modified myoblasts producing BDNF on memory of neonatal rats subjected to hypoxic-ischemic encephalopathy

目的 探讨脑内移植脑源性神经营养因子载体细胞对新生大鼠缺氧缺血性脑损伤后记忆功能的影响。方法 7d龄新生大鼠左侧颈总动脉结扎联合低氧吸入法制成缺氧缺血性脑损伤模型。损伤后即刻行左侧皮层内植入构建的稳定表达和分泌脑源性神经营养因子的大鼠成肌细胞 ,观察植入 6w后大鼠记忆功能的变化。结果 脑源性神经营养因子载体细胞脑内移植 6w后移植组动物主动回避反应习得和维持能力、一次被动回避反应的保持能力均显著提高。结论 脑源性神经营养因子载体细胞脑内移植可显著增强新生大鼠缺氧缺血性脑损伤后记忆能力的恢复和发展。

Objective To explore the effect of cerebral transplantation of genetically modified myoblasts producing brain derived neurotrophic factor (BDNF) on memory in neonatal rats subjected to hypoxic ischemic encephalopathy (HIE). Methods A unilateral transplantation of genetically engineered rat myoblast cells that were confirmed to express biologically active BDNF into the left cortex of the brain was performed shortly after HIE undergone by ligation of left common carotid artery followed by a 2.5 h inhalation of 8% O 2+92% N 2 at 37℃. Changes of memory were investigated 6 weeks after the procedure by the tests of active avoidance response, one trial passive avoidance response.Results There were significant increases of abilities of acquirement and maintenance of active avoidance response and one trial passive avoidance response in transplanted group when compared with merely injured group, although most parameters of either the two groups were lower than those of sham operated group.Conclusion Present data suggest that cerebral transplantation of genetically modified myoblasts producing BDNF promotes the recovery and enhances the development of brain memory function after HIE.