新生儿血清锌水平与T细胞亚群分布的探讨

RELATIONSHIP OF NEONATAL SERUM ZINC CONCENTRATION AND DISTRIBUTION OF T-CELL SUBSPECIES

探讨不同胎龄新生儿的血清锌水平与T细胞亚群分布的关系。应用原子吸收法测定血清锌 ;流式细胞仪测定T淋巴细胞亚群。结果显示 :2 8～ 32周早产儿组 (Ⅰ组 )血清锌水平明显低于 33～ 36周早产儿 (Ⅱ组 )及足月儿组 (Ⅲ组 ) (P <0 0 5 ) ,且Ⅰ组低锌发生率为 35 %。Ⅰ组T淋巴细胞亚群CD4+ CD8+比率明显低于Ⅱ、Ⅲ组 (P <0 0 5 ) ;Ⅰ、Ⅱ组淋巴细胞及T淋巴细胞绝对值明显低于Ⅲ组 (P <0 0 5 )。 12例低血锌新生儿CD4+T细胞水平明显低于正常组 ,CD8+T细胞水平明显高于正常组 (P <0 0 5 ) ,CD4+ CD8+比率明显下降 ,显著低于正常组 (P <0 0 0 1)。提示血清锌水平与胎龄有关 ,胎龄越小 ,锌水平越低 ,缺锌的发生率越高 ;而T细胞亚群分布不仅与胎龄有关 ,与锌水平高低也密切相关。建议加强对≤ 32周的早产儿进行锌水平的监测。

To define the relationship of neonatal serum zinc concentration and distribution of T-cell subspecies, 47 preterm and 20 term babies were enrolled and devided into 3 groups according to their gastational age (GA). Group Ⅰ: GA at 28-32 weeks, Group Ⅱ:GA at 33-36 weeks and Group Ⅲ include all of the term neonates that GA at 37-41 weeks. The blood samples after born and before eating milk were obtained and serum zinc concentration massured by atomic absorption method and T-cell subspecies by flow cytometry. Results showed serum zinc concentration of Group Ⅰ(77±24μg/dl) is significantly lower than that of Group Ⅱ (94±27μg/dl, P<0.05), and Group Ⅲ (94±16μg/dl, P<0.05),the incidence of low zinc concentration was 35%. Rate of T-cell subspecies CD4+/CD8+ ratio of group Ⅰ (1.45±0.53) was significantly lower than that of Group Ⅱ (1.78±0.47,P<0.05) and Group Ⅲ (1.99±0.99,P<0.05); lymphocyte and T-cell account of Group Ⅰ (4.61±1.80×10 9/L; 2.81±1.23×10 9/L) and Group Ⅱ (5.05±1.68×10 9/L; 2.95±1.34×10 9/L) were significantly lower than that of Group Ⅲ (6.00±1.36×10 9/L; 3.8±0.93×10 9/L, P<0.05), CD4+ T-cell level of 12 low zinc concentration cases (33.51±8.78%) lower than normal neonate Group (40.03±11.09%, P<0.05), and CD8+ T-cell level (28.8±6.93%) was higher than that of normal baby group (23.22±7.70%, P<0.05),the rate of CD4+/CD8+ decreased apparently (1.22±0.37) and lower significantly than that of normal Group (1.86±0.72, P<0.001). Therefore, it showed that a good relationship between serum zinc concentration and GA, the smaller the GA, the lower of the serum zinc concentration, the higher the incidence of low serum zinc concentration. On the other hand, there is also a good relationship between distribution of T-cell subspecies and GA and the serum zinc concentration. Suggestion was made that serum zinc concentration of preterm babies should be monitored, especialy smaller than 32 weeks.