小儿幽门螺杆菌感染与胃粘膜上皮细胞凋亡

Gastric epithelial cell apoptosis and Fas gene in children with H.pylori associated chronic gastritis

目的 探讨幽门螺杆菌 (H .pylori)相关性小儿慢性胃炎胃粘膜上皮细胞凋亡和相关基因 Fas的变化 .方法 采用 TUNEL技术和 Envision免疫组化方法检测 19例 H .py-lori阴性慢性胃炎和 17例 H.pylori阳性慢性胃炎患儿 H.pylori清除前后胃粘膜上皮细胞凋亡和相关基因 Fas的变化 .结果 19例 H.pylori阴性小儿胃炎胃粘膜上皮细胞凋亡指数和 Fas指数分别为 (2 .7± 0 .8) %和 (11.7± 9.0 ) % ;17例 H.pylori阳性小儿慢性胃炎胃粘膜凋亡指数和 Fas指数分别为 (8.5± 1.8) %和 (31.3± 1.9) % ;H.pylori阳性组凋亡指数和 Fas指数明显高于 H .pylori阴性组 P<0 .0 1. H .py-lori清除后胃粘膜上皮凋亡指数和 Fas指数均明显减少(P<0 .0 1) ,治疗前凋亡指数和 Fas指数分别为 (8.6± 2 .3) %和 (32 .6± 12 .7) % ;清除 H.pylori后 ,凋亡和 Fas指数分别为 (3.6± 1.8) %和 (13.3± 6 .4) % .结论 H .pylori感染促进胃粘膜上皮细胞的凋亡 ;Fas基因过度表达可能是 H.py-lori诱导胃粘膜上皮细胞凋亡增加的机制之一 .

AIM To investigate the change of gastric epithelial cell apoptosis and Fas gene in children with H.pylori associated chronic gastritis. METHODS Gastric epithelial cell apoptosis and Fas gene of thirty six chronic gastritis children (19 H.pylori negative and 17 H.pylori positive before and after anti H.pylori treatment) were studied by TUNEL and Envision Immunohistochemical staining. RESULTS Apoptotic and Fas indexes of 19 H.pylori negative children were (2.7±0.8)% and (11.7±9.0)% respectively; Apoptotic and Fas indexes of 17 H.pylori positive children were (8.5±1.8)% and (31.3±11.9)% respectively. The difference was significant ( P <0.01). Apoptotic and Fas of 15 H.pylori positive gastritis patients were (8.6±2.3)% and (32.5 ±12.7)% before eradication, and were (3.6±1.8)% and (13.3 ±6.4)% after eradication. The apoptotic and Fas index decreased significantly following H.pylori eradication ( P <0.01). CONCLUSION H.pylori infection might enhance gastric mucosa cell apoptosis. Super expression of Fas gene may play a key role in inducing opoptosis of gastric mucosa of H.pylori related chronic gastritis in children.