大型综合性医院524株细菌的分离鉴定及其耐药性分析

Study of the identification and resistance of 524 strain bacteria isolated in large general hospital

目的 调查分析综合性医院常见菌株对各种常用抗生素的耐药状况 ,为合理使用抗生素控制感染提供依据。方法 用K -B纸片扩散法进行药敏试验 ,试验结果用VITEK全自动细菌分离仪分离鉴定细菌并应用WHONET -4软件对临床分离菌的药敏结果进行统计分析。结果 分离出的菌株位居前 6位的依次为大肠埃希氏菌、铜绿假单胞菌、金黄色葡萄球菌、凝固酶阴性葡萄球菌、肠球菌、肺炎克雷伯氏菌。检测它们对 13种抗生素的耐药性 ,结果显示革兰氏阴性球菌除肠球菌对万古霉素耐药率有所上升 ( 9 1% )外 ,未见有其他耐万古霉素的葡萄球菌株出现。革兰氏阴性杆菌 (包括铜绿假单胞菌 )仍对泰能、头孢他啶、丁胺卡那最为敏感。耐苯唑西林的葡萄球菌对绝大多数抗生素的耐药率均在 5 0 0 %以上。大肠埃希氏菌对环丙沙星的耐药率呈直线上升 ,已达 6 3 9%。结论 定期系统地进行细菌耐药性监测对临床合理用药十分必要。

Objective To study distribution of isolated flora and flora resistant characters in general hospital for providing basis in respect of reasonable application of antibiotics.Methods Appling WHONET-4 Software in personal computer to analyze result of antibiotic sensitive test.Results First 6 kind of isolated bacteria flora arrangement in order of precedence is eschorichia coli,pseudomonas,staphylococcus aureus,staphylococcus apiolermis,enterococcus faecalis,klebsiela pneumoniae investigationg their resistant characters for 13 kind of antibiotics.The result indicated that most of above mentioned G - bacteria flora were more sensitive to IMP,CAZ,AMK in order of precedence,resistance rate of staphylococcus flora for OXA was above 50 percent,rate of escherichiacoli for CIP had been increased sharply(63 9%).Resistant rate of enterococcus VAN were 9 1%.Conclusion Monitoring of bacteria resistance systematicly and regularly is important for clinical reasonable application of antibiotics.