肺动脉高压大鼠肺动脉平滑肌细胞1,4,5-三磷酸肌醇受体表达的变化

Changes in 1,4,5-trisphosphate receptor expression in pulmonary artery smooth muscle cells of pulmonary hypertensive rats

目的 :检测肺动脉高压 ( PAH)大鼠肺动脉平滑肌细胞 ( PASMCs)上 1,4,5三磷酸肌醇受体 ( IP3 R)的亚型及 IP3 R各亚型在 PAH时表达水平的变化。方法 :采用大鼠一次性腹腔注射野百合碱 ( MCT) 6 0 mg/kg复制 PAH动物模型 ,用 Western blot法检测 IP3 R亚型的表达及在 PAH状态下其蛋白水平表达的变化。结果 :大鼠 PASMCs共同表达 3个 IP3 R亚型 ,在 PAH时 IP3 R1表达水平明显增高 ,约为对照组的 2倍 ( P<0 .0 1) ,而 IP3 R2和 IP3 R3的表达水平无明显变化。结论 :PASMCs的 IP3 R1表达增强可能参与了

Objective:To investigate the subtypes of 1,4,5trisphosphate receptor (IP 3R) in pulmonary artery smooth muscle cells (PASMCs) and changes in their expression from monocrotalineinduced pulmonary hypertensive (PAH) rats.Methods:Rats were treated with single intraperitoneal injection of monocrotaline (60 mg/kg) to produce PAH animal model and killed 21 days later.Western blot was used to identify the exact subtypes of IP 3R expressed in PASMCs and changes in their expression under PAH condition.Results:Three subtypes of IP 3R were coexpressed in rat PASMCs.The levels of IP 3R1 in PAH group was increased by 2fold without any obvious changes in IP 3R2 and IP 3R3.Conclusions:The increment of IP 3R1 expression may contribute to the pathological mechanism underlying PAH formation.