缺血和再灌注心肌NHE-1mRNA表达的变化

Change in NME -1 mRNA Expression of Rat Myocardium under Ischemia and Reperfusion

目的:探讨缺血再灌注心肌钠氢交换蛋白-1(NHE-1)mRNA表达的变化。方法:成年大鼠心脏Langendorff离体灌流,随机分为六组(n=6),给予(1)正常灌流30min;(2)低灌流缺血60min再灌注30min;(4)预处理后低灌流缺血60min;(5)预处理后低灌流缺血60min再灌注30min;(6)老龄大鼠离体心脏低灌流缺血60min等不同条件处理。保留心肌,使用RT-PCR方法对比不同处理条件下的心肌NHE-1表达的变化。结果:(1)缺血组心肌(I)NHE-1mRNA水平(0.734±0.053)较正常对照组(N)(0.300±0.007)明显升高(P<0.05),再灌注组心肌(R)NHE-1的mRNA水平(0.281± 0.019)较缺血组降低(P<0.05),与正常组无明显差异(P>0.05);(2)预处理缺血组心肌(Pi)NHE-1mR-NA水平(0.256±0.011)较缺血组心肌(0.734±0.053)明显降低(P<0.05),预处理再灌注组心肌(Pr)NHE-1mRNA水平(0.135±0.011)较再灌注组心肌(0.281±0.019)降低(P<0.05);(3)老龄大鼠低灌流缺血心肌(S)NHE-1的mRNA表达(0.787±0.021)与成年大鼠低灌流缺血心肌(I)NHE-1的mRNA表达无明显统计学差异。结论:低灌流缺血心肌NHE-1表达较正常灌流心肌明显升高,预处理可以明显降低缺血及再灌注心肌NHE-1的表达。

Objective: Na+/H+ exchanger isoform 1 (NHE - 1) plays an important role in the setting of ischemia/reperfusion injury. The aim of current study is to find out whether the mRNA expression of NHE - 1 in isolated rat hearts changes under certain pathologic conditions. Method: Langendorff perfused isolated Wistar rat hearts were randomed into six groups (n = 6): (1) 30min of normal perfusion; (2)60min of low flow ischemia; (3)60min of low flow ischemia and 30min of reperfusion; (4)60min of low flow ischemia after preconditioning; (5)50min of low flow ischemia and 30min of reperfusion after preconditioning; (6)60min of low flow ischemia of senile rat hearts. And RT - PCR was used to access the NHE - 1 mRNA expression. Result: An increase of NHE - 1 mRNA expression was observed after tow flow ischemia but it was decreased in the preconditioning/ischemia rat hearts. And the NHE - 1 mRNA expressions of rat hearts with reperfusion were higher than the reperfused hearts with preconditioning. Conclusion: The NHE - 1 mRNA expression in isolated rat heart increased under lew flow ischemia and decreased with preconditioning.