大鼠短间隔连续部分肝切除后ADAMs mRNA表达分析

Analysis of ADAMs mRNA Expression Following Short Interval Successive Partial Hepatectomy in Rat Liver

目的 说明ADAMs(adisintegrinandmetalloproteinase)在肝再生的不同阶段表达不同。方法 用RT -PCR方法和ADAMs通用引物对本实验建立的大鼠短间隔连续部分肝切除 (shortintervalsuccessivepartialhepatectomy ,SISPH )模型 0、4、8、12hr ,0、36、72、10 8、14 4hr及 0、4、40、76、112hr肝再生过程中的mRNA进行分析。结果 正常大鼠和SISPH后大鼠肝脏均存在ADAMs的表达 ,但表达强度不同。

Aim To suggest that ADAMs perform different functions in different stage in liver regeneration.Methods RT-PCR was used to analyze the expression of mRNA in three models, 0?4?8?12 hr,0?36?72?108?144 hr and 0?4?40?76?112 hr short interval successive partial hepatectomy (SISPH) with ADAMs general primers.Results ADAMs expressed in control and treated rat liver, but expression degree were different.Conclusions ADAMs may perform regulative functions during liver regeneration.