人牙胚发育过程中Smad 2分子的表达与分布变化

The Expression and Distribution of Smad 2 in Human Tooth Germ

目的 观察转化生长因子 β(transforminggrowthfactor β ,TGF β)特异的细胞内信号转导分子Smad 2在人牙胚发育过程中的表达与分布变化 ,探讨Smad 2在牙发育过程中的作用。方法 制备人牙胚发育各期标本 ,免疫组化方法检测Smad 2分子的表达。结果 Smad 2在牙胚发育的各个时期存在特异的时空分布模式 ,其分布与TGF β有相似之处。结论 首次观察到Smad 2信号分子在人牙胚发育过程中的表达 ,Smad 2作为TGF β细胞内信号分子 ,可能参与上皮 -间充质的信号诱导 。

Objective To investigate the expression of Smad 2 gene in human tooth germ,and the possible function of Smad 2 during human tooth germ development.Methods Immunohistochemical staining on prepared specimens of different stages of developing human tooth germ.Results Smad 2 expression had specific temporal spatial pattern during tooth germ development,which was similar to TGF β.Conclusion The expression of Smad 2 gene in tooth germ was detected in different stages of human tooth germ. The results suggest that Smad 2, one of the intracellular downstream molecules of TGF β , and as an inductive signal mediating epithelial mesenchymal interaction, may regulate the differentiation of ameloblast and odontoblast cells, and modulate dentinogenesis and amelogenesis.