内镜下软骨细胞移植修复颞下颌关节面软骨缺损的实验研究

An Experimental Study on Arthroscopic Chondrocytes Transplantation for Repair of Osteochondral Defects in TMJ

目的 探讨经关节镜植入软骨细胞修复颞下颌关节骨软骨缺损的可行性和修复组织的质量。方法 经关节镜对 12只猕猴的髁突和 (或 )关节结节进行钻孔 ,造成骨软骨缺损模型 ;将软骨细胞悬液与人工基质材料Pluronic F12 7混合形成复合物 ,经关节镜注入颞下颌关节面缺损区 ;4周、8周、12周和 2 4周后经大体形态观察、组织学观察、免疫组织化学染色评价修复情况。结果 软骨细胞移植术后 ,关节面缺损均得到了修复 ,修复组织内为分化良好的软骨细胞 ,并随时间推移排列逐渐有序 ;免疫组化染色发现修复组织Ⅱ型胶原染色明显。结论 关节镜导向下的细胞移植术是切实可行的。软骨细胞移植能修复关节面骨软骨缺损 ,修复组织接近正常的关节软骨组织。

Objective To certify the feasibility of arthroscopic chondrocytes transplantation in temporomandibular joint(TMJ) and the nature of repaired tissue with autologous chondrocytes transplantation in osteochondral defects. Methods The animal models of osteochondral defects in TMJ were established in 12 rhesus monkeys. Autologous auricular chondrocytes were transplanted to the defects by arthroscope.Healing of the defects was assessed by gross examination, light microscope, immunohistochemical staining. Results The repaired tissues with chondrocytes transplantation were fibrocartilages. Conclusions The technique of arthroscopic chondrocytes transplantation is feasible and the nature of repaired tissue is closest to the normal cartilage, compared with those of subchondral drilling and cartilage transplantation.