围术期胃癌患者血小板膜糖蛋白的免疫电镜观察

OBSERVATION OF PERIOPERATIVE EXPRESSION OF PLATELET GLYCOPROTENIN CD_( 41) AND CD_(62P) IN GASTRIC CANCER BY IMMUNE ELECTRON MICROSCOPY

目的 :研究胃癌患者围术期血小板膜糖蛋白 CD4 1 、CD6 2 P含量的变化和分布位置的变化 ,为进一步研究肿瘤细胞的生长、转移与血小板膜糖蛋白 CD4 1 、CD6 2 P形态、结构变化的关系奠定基础 ,为围术期防凝抗凝提供客观依据。方法 :应用免疫电镜技术检测 9例胃癌患者围术期 CD4 1 、CD6 2 P表达的变化 ,同时选择年龄相当的正常人 5例作对照。结果 :与正常人相比 ,胃癌患者 CD4 1 、CD6 2 P表达增多 ,手术使血小板活化更为加剧 ,同时观察到 CD4 1 、CD6 2 P明显的形态、结构的改变。结论 :胃癌患者血小板膜糖蛋白 CD4 1 、CD6 2 P表达和释放增加 ,处于激活状态 ;手术促使血小板活化进一步加强。

Objective:To observe the preoperative changes of human platelet glycoprotein CD 41 and CD 62P in gastric cancer,aiming to laying the foundation for studying the relation between the changes of formation and constitution of CD 41 and CD 62P and cancerous growth and transition,meanwhile,maintaining the basis for the prevention of thrombotic disease after operation.Methods:Immune electron microscopy was applied to study the changes of CD 41 and CD 62P in 9 cases of gastric cancer and in 5 cases of normal group.Results:Compared with normal group,the contents of CD 41 and CD 62P in gastric cancer were increased,and got more during operation,especially got most in 30 minutes after operation.The changes of CD 41 and CD 62P were observed not only in quantity but also in formation and constitution.Conclusion:Platelets are activated in patients with gastric cancer before operation.Operation can further activate human platelets.