前列腺癌生物学行为与端粒酶活性表达的相关性研究

RELATIONSHIP BETWEEN TELOMERASE ACTIVITY AND THE BIOLOGICAL BEHAVIOR OF PROSTATE CANCER

目的 :研究前列腺癌组织中端粒酶活性表达与前列腺癌生物学行为的关系。方法 :采用端粒重复序列扩增文件—酶标法 (TRAP- EL ISA)测定前列腺癌组织中端粒酶活性。结果 :10例正常前列腺组织无端粒酶活性。 15例前列腺癌组织中 13例检出端粒酶活性 ,阳性率 86 .0 %。端粒酶活性与肿瘤分化程度相关。 12例良性前列腺增生组织中 2例检出端粒酶活性 ,阳性率为 17.0 % ,其水平显著低于肿瘤组织。结论 :端粒酶活性与前列腺癌的生物学行为相关 ,能估测前列腺癌的生物恶性潜力。

Objective:To investigate the relationship between telomerase activity and the biological behavior of prostate cancer.Methods:The telomerase activity in prostate cancer tissues was detected by TRAP (telomeric repeat amplification protocol) ELISA which is based upon amplification of the initial telomerase product and detected by ELISA.Results:None of the 10 samples from normal prostates expressed telomerase activity.The thirteen of fifteen cases (86.0%) with prostate cancer presented telomerase activity.The activity of telomerase was associated with the pathological differentiation of prostate cancer.The two of twelve cases (17.0%) with benign prostatic hyperplasia (BPH) expressed telomerase activity,and the activity of telomerase was lower than prostate cancer.Conclusion:The telomerase acitivity may be related to the biological behavior of prostate cancer and can evaluate the malignant potential of prostate cancer.The presence of telomerase acitivity in some cases of BPH may indicate the presence of a minority of malignant cells,as yet clinically undetected,within the population of BPH cells.