p16、p27蛋白在骨巨细胞瘤和骨肉瘤中的表达

EXPRESSION OF p16 AND p27 IN HUMAN GIANT CELL TUMORS OF BONE AND OSTEOSARCOMAS

目的 :探讨抑癌基因 p16、p2 7在骨巨细胞瘤 (GCT)和骨肉瘤 (OS)中的蛋白表达特点及其在骨肿瘤发生中的意义。方法 :应用 SABC免疫组织化学方法检测 p16、p2 7蛋白在 16例骨肉瘤、4 6例骨巨细胞瘤 (其中 Jaffe 级 18例、 级 6例、 级 12例 )中的表达。结果 :p16和 p2 7蛋白的阳性表达均定位于骨肉瘤细胞、骨巨细胞瘤的基质细胞的细胞核和细胞质内。在骨巨细胞瘤 级、 级、 级和骨肉瘤中 p16蛋白的阳性表达率依次为 16 .7%、6 2 .5 %、6 6 .7%和 2 5 % ;p2 7蛋白的阳性表达率依次为 11.8%、5 6 .3%、5 0 %和 2 5 %。p16和 p2 7蛋白的骨巨细胞瘤 级中的阳性表达率显著地低于 级和 级 (P<0 .0 5 ) ,p16蛋白在骨肉瘤与骨巨细胞瘤 级之间的阳性率差异有显著性意义 (P<0 .0 5 ) ,而 p2 7蛋白在骨肉瘤和骨巨细胞 级之间的率差异则不具有显著性意义 (P>0 .0 5 )。结论 :p16、p2 7基因在骨巨细胞瘤和骨肉瘤的发生中起重要作用 ,但其阳性表达率不足以单独作为判断恶性程度和预后的指标。

Objective:To determine p16,p27 expression and their relationships in giant cell tumors of bone (GCT) and osteosarcomas (OS).Methods:Sixty two cases,including 18,16,12 and 16 cases of GCT Ⅰ,Ⅱ,Ⅲ and OS were examined by SABC immunohistochemistry methods.Results:The p16 and p27 antigen positive immunostaining located in the cytoplasms and the cellular nuclei.In GCT Ⅰ,Ⅱ,Ⅲ and OS,the detection rate of p16 were 16.7%,62.5%,66.7% and 25% respectively and the detection rates of p27 were 11.8%,56.3%,50% and 25% respectively.Statistically,the p16 and p27 positive rates between GCT Ⅱ and GCT Ⅲ were not significant ( P >0.05) while those between GCT Ⅰ and the collection of GCT Ⅱ and GCT Ⅲ were significant ( P <0.05).The positive rate difference of p16 between OS and GCT Ⅲ was significant ( P <0.05) while that of p27 was not ( P >0.05).Conclusion:The results indicated that neither the p16 positive rate nor the p27 positive rate could be used independently for defining grade of malignancy and estimating prognosis of malignant bone tumors though p16 and p27 might play important roles in the genesis of bone tumors.