内皮素-1对大鼠睾丸间质细胞睾酮分泌影响的可能作用机理

Possible Mechanisms of Endothelin-1 Influencing Testosterone Secretion of Rat Leydig Cells in vitro

目的 :了解内皮素 1(ET 1)影响大鼠睾丸离体间质细胞睾酮分泌的可能作用机理 ,为男性不育症治疗提供一定的理论基础。 方法 :采用大鼠睾丸间质细胞体外孵育技术以及12 5I 睾酮放射免疫法 ,观察ET 1对间质细胞睾酮分泌的影响及其可能作用机理。 结果 :内皮素受体A(ETA)、内皮素受体B(ETB)受体拮抗剂均可逆转ET 1对大鼠离体间质细胞睾酮分泌的抑制作用 ,但Ca2 +通道阻滞剂维拉帕米 (VER)未表现此作用。 结论 :ET 1通过与ETA及ETB结合而发挥作用。其Ca2 +的动员可能不是细胞外Ca2 +进入细胞内。

Objectives: To determine the effect of endothelin 1 on the testosterone secretion by rat Leydig cells and to discuss its possible mechanisms. Methods: After rat Leydig cells were incubated,testosterone was detected by radioimmunoassay. Results: Antagonist of endothelin receptor (ETA and ETB) could suppress the effect of ET 1 on inhibiting testosterone secretion,but Verapamile,a calcium channel blocker,could not do so. Conclusions: The study showed that ET 1 inhibits testosterone secretion of rat Leydig cells in vitro by combining with ETA and ETB.Mobilization of Ca 2+ may not flow from extracellular to intracellular.