染矽尘第30天大鼠血浆、肺组织匀浆NO、NOS的关系

The relations between NO and NOS in plasma and mix - ups of lung of rats exposed to silica dust after 30 days

[目的]探讨染矽尘大鼠血浆、肺组织匀浆NO、NOS的关系。[方法]采用气管注射染矽尘(40mg/只)方法建立动物模型;血浆、肺组织匀浆NO浓度测定采用硝酸还原酶法;血浆、肺匀浆NOS活性测定采用NOS催化L—Arg法;采用SPSS8.0统计软件进行t检验和相关分析。[结果]矽尘可导致大鼠血浆NO水平升高(P<0.05)和NOS活力降低(P<0.05)、肺匀浆NO水平(P<0.05)和NOS活力均升高(P<0.01)。实验组血浆NO和血浆NOS有负相关关系(r=-0.775,P<0.05);血浆NOS和肺匀浆NO有负相关关系(r=0.800,P<0.05)。[结论]矽尘可导致大鼠血浆、肺匀浆NO水平和NOS活力变化;矽尘可导致血浆NO和血浆NOS有负相关关系,血浆NOS和肺匀浆NO有负相关关系。

[Objective] To study the relations between NO and NOS in plasma and mix - ups of lung of rats exposed to silica dust after 30 days. [Methods] Animal models were established by injecting silica (40 mg per animal) into the lung of rats through trachea. NO level was determined with nitric acid reductase method, NOS bioactivity was determined by NOS catalyze L - Arg method. Examinations and relevant analysis were made with SPSS8.0 counting software. [Results]silica dust caused an increase in the level of plasma NO ( P <0.05) and a decrease of bioactivity of NOS in plasma ( P < 0.05) . At the same time, there was an increase in the mix - ups NO ( P < 0.05) and NOS ( P < 0.01) . There were negative relations between plasma NO and plasma NOS ( P < 0.05, r = - 0.775). This trend also existed between plasma NOS and lung mix - up NO ( P < 0.05) . [Conclusions] Silica dust may cause changes in level of NO and bioactivity of NOS in plasma and lung mix - ups of rats exposed to silica, and there were negative relations between plasma NO and plasma NOS, and this trend also exists between plasma NOSS and mix - ups NO.