鳜传染性脾肾坏死病毒核糖核酸酶III基因结构及序列分析

Characterization of the ribonuclease III gene of infectious spleen and kidney necrosis virus of Siniperca chuatsi

测定了鳜传染性脾肾坏死病毒 (ISKNV)核糖核酸酶III(RNaseIII)基因的核苷酸全序列。该基因完整读码框为 771bp ,GC含量为 5 2 .5 3% ,编码一个长为 2 5 6个氨基酸、分子量为 2 8.9kD的推定蛋白。其上下游序列各有一个TATAbox ,终止子下游有一段回文序列。与其它物种相比 ,ISKNV与虹彩病毒 (包括LCDV - 1和CIV)的核糖核酸酶III基因的氨基酸序列同源性较高 ,与酵母。

Ribonuclease III gene of infectious spleen and kidney necrosis virus(ISKNV)of Siniperca chuatsi had been sequenced. The open reading frame of the gene is 771 nucleotides long, encoding a putative protein of 256 amino acids with 28.9 kD. The 5' and 3' noncoding regions have TATA boxes. A palindrome is contained downstream of the translation stop codon. Compared with the RNase IIIs found in ISKNV and other species, the higher homology among ISKNV and the two iridovirus is present, and ISKNV has lower identity with other species, especially yeast and rhabditida.