云南烟蚜羧酸酯酶活力测定条件的研究

Preliminary Studies on Determining Condition of α-NA Carboxylesterase Activity in Yunnan Myzus persicae

比较 5种α -乙酸萘酯羧酸酯酶活力测定方法 ,发现唐振华等 (1988)方法较好。进一步以底物浓度、反应温度、温浴时间、pH作因子 ,采用正交设计的方法来确定α -乙酸萘酯羧酸酯酶活力测定的最佳条件 ,结果表明 ,除pH影响不显著外 ,其余 3个因子均影响显著吸光值 ,其中底物浓度影响极显著。 4因子的主次关系为 :底物浓度 >反应温度 >温浴时间 >pH。测定云南烟蚜的羧酸酯酶活力时 ,以底物浓度 6 0× 10 -4mol/L ,缓冲液pH 8 0 ,37℃保温

Making a comparison among five determining methods of α-NA carboxylesterase activity,one best methods was screened.The effects of substrate concentration,reaction temperature,reaction time and pH on the OD value were studied in further orthogonal test. The result shows that the former three factors all have significant effects on OD,while pH hasn't,and shows that the best determining condition is that four factors are respectively 6 0×10 -4 ?mol/L,37?℃,30?min,pH?8 0.