碳酸氢钠浸提有机磷测定方法探讨

Study on the measurment of organic phosphorus in sodium bicarbonate extracts

碳酸氢钠浸提有机磷的消煮过程是否有磷的挥发和转化 ,确定是否有必要在消煮前添加饱和MgCl2 溶液和消煮后水解 ,试验测定了焦磷酸与偏磷酸、已知浸提有机磷化合物 (核酸与苯磷酸钠 )、两种浸提液 (土壤与秸秆 )及含葡萄糖的磷酸二氢钾溶液消煮和水解后的无机磷量。结果表明 ,消煮能促进偏磷酸和焦磷酸的水解 ,消煮过程没有磷的挥发和转化 ,没有磷损失 ,消煮前无需添加饱和MgCl2 溶液 ,消煮后也不用水解。

Whether there were losses of P during digestion by volatilisation of orthophosphate or formation of metaphosphate and pyrophosphate was studied. The necessity of hydrolysis and additon of saturated MgCl 2 was discussed. The quantities of inorganic phosphorus after digestion and hydrolysis of pyrophosphate and metaphosphate, organic phosphate, two extracts and inorganic phosphate containing glucose were measured. The results showed that hydrolysis of pyrophosphate and metaphosphate was enhanced during digestion. There were no P losses during digestion. It's not necessary to add saturated MgCl 2 before digestion and hydrolyze after digestion.