不同人群丙型肝炎病毒基因分型

The genotypes of hepatitis C virus in different populations

目的 研究不同人群丙型肝炎病毒的基因分型。方法 对 141例抗HCV(+)、6 0例抗HCV(- )及 2 8例正常献血员的共 132份HCV -RNA阳性血清 ,运用限制性片段长度多态性分析 (RFLP)法作酶切分型。结果 3组人群不同基因型检出率分别是 1b Ⅱ型 87.18%、10 0 %、10 0 % ;2a Ⅲ型 1.71%、0、0 ;两者混合型 (1b 2a型 )11 11%、0、0。对抗HCV(+)血清 ,分为ALT正常组和异常组 ,2组人群不同基因型检出率分别是 1b Ⅱ型93 33%、83.33% ;2a Ⅲ型 0、2 .78% ;两者混合型 7.14%、13.89%。结论 我国不同人群中HCV感染者均以HCV1b Ⅱ型为主 ,今后应以HCV 1b Ⅱ型为重点 ,加强HCV的诊断。

Objective To investigate the genotypes of hepatitis C virus (HCV) in different groups of people.HMethods Polymerase chain reaction (PCR) was employed to test the specimens taken from 141 anti-HCV (+) cases, 60 anti-HCV (-) cases and 28 normal blood donors; and 132 out of them were found to be HCV-RNA (+).Restriction fragment length polymorphism analysis (RFLP) was used to analyse the genotype of the 132 HCV-RNA (+) cases.?Results In the anti-HCV (+) group ( n =117),genotype 1b/Ⅱ accounted for 87.18%,genotype 2a/Ⅲ for 1.71% and mixed genotype 1b/2a for 11.11%. In the HCV-RNA(+) cases ( n =15) detected from the anti-HCV (-) patients and blood donors ,the HCV were all found to be 1b/Ⅱ in genotype .When anti-HCV(+) was subdivided into normal ALT group ( n =45) and abnormal ALT group ( n =72),genotype 1b/Ⅱ accounted for 93.33% and 83.33% respectively, genotype 2a/Ⅲ for 0 and 2.78%, and mixed genotype 1b/2a for 7.14% and 13.89% respectively.?Conclusion Genotype 1b/Ⅱ is the predominant HCV that infects different groups of people in China. Hence the relevant studies on diagnosis, treatment and vaccine preparation should be focused on the genotype 1b/Ⅱ of HCV.