摘要
目的 构建新型免疫毒素BDI- 1-PEA ,并进行抗人膀胱肿瘤活性的初步研究测定。方法 应用异型双功能连接剂N -琥珀酰胺酯 3- (2 -吡啶基二硫 )丙酸 (SPDP)将抗人膀胱肿瘤单克隆抗体 (BDI- 1)与铜绿假单胞菌外毒素 (PEA)交联 ,制备出新型免疫毒素BDI- 1-PEA。应用四甲基偶氮唑盐 (MTT)法初步检测其对人膀胱肿瘤细胞系E -J的选择性杀伤活性。结果 BDI- 1-PEA结合物具有高效靶向杀伤膀胱肿瘤细胞的能力 ,优于游离PEA或PEA与BDI- 1的混合物。结论 新型免疫毒素BDI- 1-PEA有较高的选择性抗人膀胱肿瘤细胞的活性 。
Objective To construct the immunotoxin BDI-1-PEA and study its cytotoxicity against human bladder carcinoma in vitro. Methods The immunotoxin BDI-1-PEA was prepared by conjugating pseudomonas exotoxin A (PEA) with monoclonal anti-bladder cancer antibody BDI-1 by using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). Results Study in vitro indicated that BDI-1-PEA showed stronger selective cytotoxicity against human bladder carcinoma cell line E-J than free PEA or the mixture of BDI-1 and PEA. Conclusion BDI-1-PEA conjugate is an effective immunotoxin against human bladder carcinoma and is a promising candidate for further clinical study.
出处
《徐州医学院学报》
CAS
2001年第5期386-389,共4页
Acta Academiae Medicinae Xuzhou
基金
江苏省教委自然科学基金课题 (98KJB32 0 0 0 7)
