沙土鼠脑缺血后早期亚低温对行为学及组织学损害的保护作用

The protective effect of mild hypothermia on cognitive function and histological changes associated with cerebral ischemia in gerbils

目的 探讨沙土鼠脑缺血后早期亚低温干预对认知行为学及组织学损害的保护作用。方法 采用沙土鼠双侧颈总动脉无损伤扎闭法制作前脑缺血再灌注损伤模型 ,观察缺血后时程 3h的亚低温对认知行为学及组织学损害的保护作用。结果 缺血后早期亚低温对脑缺血造成的认知功能障碍有保护作用 ,特别是对近期记忆功能的改善有帮助 ,组织学检查见海马CA1区锥体细胞损害明显减轻。结论 缺血后早期亚低温对脑缺血造成的认知功能障碍及组织学损害有保护作用 。

Objective To investigate the protective effect of postischemic hypothermia on cognitive function deficits and histological damage in gerbils' cerebral ischemic model induced by bilateral common carotid artery occlusion. Methods Thirty gerbils were divided into three groups:①Control; ②Subjected to 10 minutes of forebrain ischemia at 37.5 ℃(rectal temperature) and maintained at 37.5 ℃ for 3 hours; ③Subjected to 10 minutes of forebrain ischemia at 37.5 ℃ and maintained at 33 ℃ for 3 hours. Behavioral and histological examinations were conducted 6 to 7 days after ischemia. Results ①Normothermic ischemia produced impairments in passive avoidance, spatial navigation and working memory. Postischemic hypothermia significantly attenuated cognitive deficits induced by cerebral ischemia, especially the memory function. ②There was a significant loss of CA1 pyramidal cells in the normothermic ischemic group. Neuronal damage was attenuated by 3 hours of postischemic hypothermia. Conclusions ”BPostischemic hypothermia for three hours is effective at attenuating ischemic-related histological damage and behavioral deficits.