革螨、恙螨传播肾综合征出血热病毒的实验研究

Experimental study on the roles of gasmid mite and chigger mite in the transmission of hemorrhagic fever with renal syndrome virus

目的 研究革螨、恙螨在肾综合征出血热病毒 (hemorrhagicfeverwithrenalsyndromevirus,HFRSV)传播中的媒介作用。方法 采集自然界鼠窝革螨及饲养繁殖的子代革螨和鼠体恙螨幼虫以及从小黑板采集游离恙螨幼虫及饲养孵化的若虫 ,进行螨细胞培养分别观察螨细胞传播HFRSV的作用。结果 发现螨细胞培养第 15d和第 2 0d可从螨细胞内检测到HFRSV ,其中HFRSV检出数和荧光强度在革螨子 4代、子 3代和恙螨若虫均多于和强于子 1代、子 2代及恙螨幼虫。结论 革螨、恙螨对HFRSV的传播有一定的媒介作用 ,在维持HFRSV的自然循环中起重要作用。

Objective To study the roles of gasmid mite and chigger mite in the Transmission of hemorrhagic fever with renal syndrome virus (HFRSV). Methods Natural gasmid mite,the first generation gasmid mite, chigger mite larvas from rats, free chigger mite larva and nymph were collected and their cells were cultured to study the role of transmission HFRSV. Results HFRSV was detected from mite cells after cultured for 15 and 20 days. The number of HFRSV and fluorescence intensity detected from the fhird and fourth generation gasmid mite and chigger mite nymph was much more than those from the first, second generation and chigger mite larva. Conclusion Gasmid mite and chigger mite had an important role to play on HFRSV transmission in heeping the natural cycles.