HSV-1截短gB基因DNA疫苗的构建及初步鉴定

The construction and preliminary appraisement of HSV-1 truncated gB gene DNA vaccine

目的 为有效预防单纯疱疹Ⅰ型病毒 (HSV - 1 )感染 ,给多价DNA疫苗的构建奠定基础 ,构建表达截短HSV - 1 gB糖蛋白的DNA疫苗。方法 用PCR技术从HSV - 1基因组中扩增出编码HSV 1gB糖蛋白 1 - 5 1 7氨基酸序列的一段基因序列 ,通过 pGEM -T中介载体 ,将其插入到真核表达质粒pcDNA 3 1 (+)的CMV启动子下游。结果 构建的重组真核表达质粒可在动物体内正确表达目的基因 ,对HSV - 1病毒攻击具有免疫保护作用。结论 本研究对截短HSV - 1 gB基因DNA疫苗进行了初步的探索性研究 ,也为多价HSV 1DNA疫苗的构建奠定了基础。

Objective In order to prevent infection from herpes simplex virus type 1(HSV-1), and make the found for the construction of multi-valent DNA vaccine, a truncated HSV-1 gB gene DNA vaccine was constructed. Methods By means of PCR technique, a fragment of DNA sequence which encodes the amino acid sequence 1-517 of the HSV-1 glycoprotein B was obtained from the HSV-1 genome. The fragment was then inserted into the lower stream of CMV promoter in the eukaryotic plasmid pcDNA?3.1(+) which had been aided by intermediary vector plasmid pGEMT.Results The recombinant eukaryotic plasmid could correctly express the order gene and induce an immune protection against HSV-1 in vivo .Conclusion This research has paved the way for study on truncated HSV-1 gB gene DNA vaccine, and also make the found for the construction of multi-valent DNA vaccine of HSV-1.