胰腺癌中VEGF mRNA,bFGF mRNA和PDGF mRNA及相应受体的表达与意义

Significance of the expressions of VEGF mRNA, bFGF mRNA,PDGF mRNA and their receptors in pancreatic carcinoma

目的 探讨血管内皮生长因子 (VEGF)mRNA ,碱性成纤维细胞生长因子 (bFGF)mRNA和血小板衍生生长因子 (PDGF)mRNA及相应受体 (flt 1,bFGFR1,PDGFR)与肿瘤微血管 (microres sel ,MV)生成在胰腺癌生成、侵袭、转移中的作用及它们的相互关系。方法 应用原位分子杂交技术检测 5 1例胰腺癌及 32例急、慢性胰腺炎病人病变组织中VEGFmRNA ,bFGFmRNA ,PDGFmR NA的表达 ,应用免疫组化方法检测相应病例中flt 1,bFGFR ,PDGFR的表达和肿瘤微血管计数(MVC)。分析其表达与胰腺癌病理参数及预后的关系。结果 胰腺癌中 3种血管生长因子mR NA及受体阳性率明显高于急慢性胰腺炎 (P <0 .0 5或P <0 .0 1) ,它们的高表达与胰腺癌的分化程度呈负相关 (P <0 .0 5 ) ;与肿瘤的浸润、淋巴及血道转移 (肿瘤的进展阶段 )呈正相关 (P<0 .0 5或P <0 .0 1) ,而与肿瘤大小无关 ;癌组织中MVC明显高于急慢性胰腺炎 (P <0 .0 1) ;3种血管因子及受体与胰腺癌MVC呈正相关 (P <0 .0 5或P <0 .0 1) ;癌组织中 3种血管因子mRNA及bFGFR与术后生存率呈显著负相关 (P <0 .0 1)。结论 VEGFmRNA ,bFGFmRNA ,PDGFmRNA及受体的表达及MVC的测定可作为判定胰腺癌生长、转移及预后的重要指标 ,阻断

Objective To study the effect of intratumor angiogenesis and vascular growth factor(VEGF), basic fibroblase growth factor(bFGF), Platelet-derived growth factor (PDGF), and their receptors(flt-1 bFGFR.PDGFR) on the invasion, metaslasis of pancreatic carcimoma(PC) and the relationship between the expressions of three kinds of angiogenic factors, their receptors and microvessel count(MVC). Methods Tis- sue sections of 51 PC and 32 acute or chronic pancreatitis were examined by in situ hybridization for the expression of VEGF bFGF.PDGF, and by immunohistochemistry for the expression of the three kinds of angiogenic factor receptors and MVC. The correlation of the expressions and pathological characteristics of PC were also studied. Results The positive rate of VEGF mRNA,bFGF mRNA, PDGF mRNA and their receptors in PC were significantly higher than that in acute or chronic pancreatistis( P <0.01 or P <0.05). The high positive expression of the 3 factors and their receptors had significant negative correation with the differentiation degree ( P <0.05), and with regional invasion lymhatic blood streem metastasis ( P <0.01 or P <0.05), had positive correation with regional invasion lymphatic and blood streem metastasis ( P <0.01 or P <0.05) in PC, and no correlation with the size of PC. MVC in cancer tissues was higher than that in acute of chronic pancreatits ( P <0.01). Overexpression of three kinds of angiogenic factors and their receptors had positive correlation significantly with MVC ( P <0.01 or P <0.05). The overexpression of VEGF mRNA,bFGF mRNA,PDGF mRNA, bFGFR had poor survival rate ( P <0.01). Conclusions The expressions of VEGF mRNA, bFGF mRNA, PDGF mRNA and Their receptors are highly correlated with MVC, and with the growing invasion and melastasis of PC, probably as a parametor for determining the metastosis and prognosis of PC.