免疫耐受对日本血吸虫虫卵肉芽肿形成的影响

EFFECTS OF IMMUNO-TOLERANCE ON THE FORMATION SCHISTOSOMA JAPONICUM EGG GRANULOMA IN MICE

目的 探讨免疫耐受对小鼠肝脏虫卵肉芽肿形成的影响。 方法 分别用 10、10 0、和 10 0 0 μg的日本血吸虫可溶性虫卵抗原 (SEA)腹腔注射 1周龄小鼠 ,诱导其对虫卵抗原产生免疫耐受性。在感染日本血吸虫尾蚴后 42、5 6、70和 84d分批剖杀小鼠 ,取出肝脏作病理切片测量肉芽肿的大小。 结果 10 0～ 10 0 0 μg SEA可诱导小鼠产生一定程度的免疫耐受性 ,使虫卵肉芽肿反应减轻。但是随着时间的延长 ,这种耐受性逐渐减弱或消失。 结论 SEA诱导的免疫耐受性可以减轻血吸虫感染早期的肝脏肉芽肿反应 ,但对慢性期的作用较弱。

Objective To study effects of immuno tolerance on egg granuloma formation in mice infected by Schistosoma japonicum . Methods S. japonicum soluble egg antigen (SEA) was administered i.p. at a concentration of 10, 100 or 1 000 μg protein in 1 week old mice. Mice were sacrificed respectively at 42, 56, 70 and 84 days after S. japonicum cercaria infection. The liver was removed for histopathological study. Results The proper concentration of SEA (100-1 000 μg) may induce certain level of SEA specific immuno tolerance and reduction in granuloma diameter in treated mice from 42 to 70 days after infection. The tolerance gradually relented or disappeared from 70 days after infection. Conclution Immuno tolerance induced by SEA may reduce host hepatic egg granulomatous reaction around S. japonicum eggs and the effect was more obvious in early stage of schistosomiasis japonicum than in chronic stage of schistosomiasis japonicum.