RAPD在判别肺炎克雷伯菌暴发流行中的应用

Applying RAPD Technique to Study the Epidemiology of Klebsiella Pneumoniae

肺炎克雷伯菌是重要的条件致病菌 ,常常引起老人及婴幼儿重症护理病房的交叉感染及暴发流行 ,追踪其传染源尤为重要 ,故多年来许多学者致力于细菌的克隆识别研究。 1990年 ,Williams和Welsh几乎同时提出了一种遗传标记技术———随机引物PCR(RAPD) [1,2 ] 。本人于同一时期从两个病房共收集 7株肺炎克雷伯菌 ,经RAPD分析后 7株菌出现两个型。联合RAPD分析和表型分析证实肺炎克雷伯菌有流行趋势。

Klebsialla pneumoniae is an important hospital-acquired pathogen that causes severe morbidity and mortality in elder patients and infant patients.For along time,many researchers have been founding efficient method of identifying clone.In 1990,Williams and Welsh proposed a new technique of genetic marker,that was randomly amplified polymorphic DNA(RAPD).7 kinds of pneumoniae strains were collected from two wards of a hospital at the same time,and RAPD fingerprints of strains showing 7 strains to be two patterns of the epidemic strain.The combination of RAPD typing and the phenotyping techniques,we can conclude that an outbreak occurs.