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RAPD在判别肺炎克雷伯菌暴发流行中的应用 被引量:1

Applying RAPD Technique to Study the Epidemiology of Klebsiella Pneumoniae
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摘要 肺炎克雷伯菌是重要的条件致病菌 ,常常引起老人及婴幼儿重症护理病房的交叉感染及暴发流行 ,追踪其传染源尤为重要 ,故多年来许多学者致力于细菌的克隆识别研究。 1990年 ,Williams和Welsh几乎同时提出了一种遗传标记技术———随机引物PCR(RAPD) [1,2 ] 。本人于同一时期从两个病房共收集 7株肺炎克雷伯菌 ,经RAPD分析后 7株菌出现两个型。联合RAPD分析和表型分析证实肺炎克雷伯菌有流行趋势。 Klebsialla pneumoniae is an important hospital-acquired pathogen that causes severe morbidity and mortality in elder patients and infant patients.For along time,many researchers have been founding efficient method of identifying clone.In 1990,Williams and Welsh proposed a new technique of genetic marker,that was randomly amplified polymorphic DNA(RAPD).7 kinds of pneumoniae strains were collected from two wards of a hospital at the same time,and RAPD fingerprints of strains showing 7 strains to be two patterns of the epidemic strain.The combination of RAPD typing and the phenotyping techniques,we can conclude that an outbreak occurs.
机构地区 燕山大学
出处 《齐齐哈尔医学院学报》 2001年第6期606-607,共2页 Journal of Qiqihar Medical University
关键词 随机引物 RAPD 肺炎克雷伯菌 流行病学 Randomly amplified polymorphic DNA(RAPD) Klebsiella pneumoniae Epidemiology research
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参考文献3

  • 1[1]Williams, J.G.K. , A.R Kubelik,et al. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Rds, 1990,18:6 531~6 535
  • 2[2]Welsh, J, M. Meclelland. Fingerprinting genomes using PCR with arbitrarily primes. Nucleic Acids Res, 1990,18: 7 213~ 7 218
  • 3[3]Elaichouni A, Verschraegen G, Claeys G, et al. pseudomonas aeruginosa serotype O12 outbreak studied by arbitrary primer PCR. J Clin Microbiol, 1994,32:666

同被引文献6

  • 1Kluytmans J, Berg H, Steegh P, et al. Outbreak of staphylococcusschleiferi wound infections: Strain characterizationby randomly amplified polymorphic DNA analysis, PCR ribotyping,conventional ribotyping, and pulsed-field gelelectrophoresis[J].J Clin Microbiol,1998,36(8):2214-2219.
  • 2Martin MC, Fueyo JM, Gonzalez Hevia MA, et al. Geneticprocedures foridentification of enterotoxigenic strains of Staphylococcus aureus from three food poisoning outbreaks[J].Int J Food Microbiol,2004,94(3):279-286.
  • 3Olmos A, Camarena JJ, Nogueira JM. Application of anoptimized and highly discriminatory method based onarbitrarily primed PCR for epidemiologic analysis ofmethicillin-resistant Staphylococcus aureus nosocomialinfections[J]. J Clin Microbiol,1998,36(6):1128-1134.
  • 4Ben-Hamouda T, Foul on T, Ben-Cheikh-Masmoudi A, etal. Molecular epidemiology of an outbreak of multiresistantKlebsiella pneumoniae in a Tunisian neonatal word[J].JMed Microbiol,2003,52(5):427-433.
  • 5方会龙,曾庆仁,王俊杰,曾铁兵,喻容,杨胜辉,刘碧源,刘彦,蔡力汀.运用Quantity One软件分析日本血吸虫不同发育时期蛋白组分差异[J].中国血吸虫病防治杂志,2010,22(4):320-323. 被引量:6
  • 6叶明亮,袁著忻,吴海寰.8种细菌随机扩增多态性DNA引物筛选与反应体系优化[J].中华检验医学杂志,2002,25(2):114-115. 被引量:12

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