解整合素-金属蛋白酶12基因在骨巨细胞瘤组织中的表达及意义

Expression of ADAM12 (Meltrin α) gene in giant cell tumor of bone

目的 检测解整合素 金属蛋白酶 12 (ADAM12 )基因在骨巨细胞瘤组织中的表达和定位 ,探讨其对骨巨细胞瘤中多核巨细胞形成的作用。方法 用逆转录 聚合酶链反应 (RT PCR)检测18例、用RNA原位杂交检测 12例骨巨细胞瘤患者的瘤组织、6例体外培养骨巨细胞瘤瘤细胞、2例胚胎横纹肌和 5例成人横纹肌组织的ADAM12mRNA。结果 RT PCR显示 ,18例骨巨细胞瘤组织中 ,12例 (6 7% )有ADAM12mRNA表达 ;RNA原位杂交则显示 12例骨巨细胞瘤组织全部呈ADAM12阳性反应 ,并且位于几乎所有的多核巨细胞和单核基质细胞胞质中。随着骨巨细胞瘤培养细胞传代次数的增多和多核巨细胞的消失 ,ADAM12mRNA的表达也逐渐消失。结论 骨巨细胞瘤中的多核巨细胞可能是由单核基质细胞融合而成 ,ADAM12基因参与了这一融合过程。

Objective To detect expression and location of ADAM12 (meltrin α) gene in giant cell tumor of bone and to study its effect on the formation of multinucleated giant cells in the tumor. Methods ADAM12 mRNA was detected by RT PCR and RNA in situ hybridization in 18 cases of giant cell tumors, 6 cases of cultured tumor cells, 2 specimens of embryonic muscle tissue and 5 specimens of adult muscle tissue. Results RT PCR showed that 12/18 (67%) cases of giant cell tumor of bone expressed ADAM12 mRNA. RNA in situ hybridization demonstrated positive ADAM12 mRNA in 12 cases of the detected tumors. The ADAM 12 mRNA positive signal was localized in the cytoplasm of almost all multinucleated giant cells and mononuclear stromal cells. However, the expression of ADAM12 mRNA decreased gradually, and eventually lost with increased passages of tumor cells and the disappearance of multinucleated giant cells in the culture. Conclusion Multinucleated giant cells probably originated from the fusion of mononuclear stromal cells and ADAM12 may participate in the fusion process.