硼酸基亲和层析纯化5-甲基尿苷

Purification of 5-Methyluridine by Boronate Affinity Chromatography

利用聚乙烯系大孔聚合物与间氨基苯硼酸偶联制备亲和介质 ,配基密度为每克湿胶 0 .92mmol。最佳吸附条件为 p H9.0和 2 .0 mol/ L Na Cl;最佳洗脱条件为 0 .1 mol/ L HCl。测定了吸附等温线 ,算得解离常数 Kd 为 0 .1 5 5 mol/ L,最大吸附量 qm 为每克湿胶 78.8mg。将含有胸腺嘧啶的5 -甲基尿苷溶液进行亲和层析 ,5 -甲基尿苷收率为 5 6.3%

The affinity adsorbent was prepared by reacting an epoxy containing polyvinyl polymer with meta aminophenylboronic acid ( m APBA). The adsorbent has a ligand density of 0.92mmol/g wet gel. The optimum pH and the optimum ionic strength for 5 methyluridine(5 MUR) adsorption are 9.0mol/L and 2.0 mol/L NaCl, respectively. The optimal condition for elution is 0.1mol/L HCl. The adsorption isotherm of 5 MUR on the medium was determined, from which the dissociation constant K d of 0.155 mol/L an...