血中神经性毒物和有机磷农药的气相色谱分析

Gas Chromatographic Analysis of the Trace Nerve Agents and Organophosphorus Pesticides in Blood

从生物体液中分离检定神经性毒物和有机磷农药有溶剂提取法,但选择性差,需与高度专的分析方法(如气相色谱-质谱联用)相结合;另外还有竞争抑制酶免疫测定法,^(32)P标记放射测定法及胆碱酯酶抑制测定等方法,以上方法操作复杂。

This method uses Sep- Pak C18 cartridge to concentrate trace nerve agents and organophosphorus pesticides in blood. Methano! was selected as the eluating solvent. The eluates were analyzed on Varian-6000 Gas Chromatograph equipped with nitrogen/ phosphorus detector (NPD). The recoveries were in the range of 65 -99%. In this way the minimum detectable concentration was 10pg/ml blood. The method is simple, rapid and highly sensitive. It is shown experimentally that the Sep-Pak C18 cartridge may be replaced by X- 5 resin cartridge.