乙酰谷氨酰胺锌抗实验性胃溃疡的作用研究

Antiulcer activities of acetyl-glutamine Zinc on Gastric lesion models

目的 研究新化合物乙酰谷氨酰胺锌 (Zn GL)对大鼠实验性胃溃疡的防治作用及机制。方法 分别采用大鼠水浸应激性胃溃疡 ,无水乙醇、吲哚美辛和慢性醋酸诱导的胃溃疡为实验模型。以胃黏膜损伤程度 ,溃疡指数及胃壁结合黏液含量为观察指标。结果 1 0 0或 1 50mg·kg- 1Zn GL灌胃给药对 4种大鼠急慢性胃黏膜损伤均有明显的预防或治疗作用 ,胃溃疡指数显著降低 ,胃液分析显示该化合物能显著升高胃壁结合黏液含量 ,光镜下观察未见胃黏膜组织炎性细胞浸润。结论 该化合物具有较强的抗溃疡作用 ,这一作用依赖于内源性的PGE2 ,并与加强胃黏液屏障防御功能 ,促进溃疡愈合有关。

OBJECTIVE: To study the therapeutic and preventive effects of the new compound acetyl-glutamine zinc(Zn-GL) on gastric ulcer models. METHODS: The gastric ulcer was induced by intragastric alcohol, subcutaneous indomethacin(40 mg&middotkg-1), cool water immersion and chronic acetate, respectively. The gastric injury was evaluated microscopically and histologically. The general acidity of the contents in stomach was assessed and the mucus secretion was determined by Alcian blue assay. RESULTS: It was found that 100 or 150 mg&middotkg-1 Zn-GL given orally protected markedly against gastric mucosal lesion formed in the four experimental models. In regard to indomethacin induced and chronic acetate ulcers, Zn-GL showed stronger inhibition than that of cimeditine at the dosage higher than the former. In addition, the gastric mucus contents were significantly elevated by Zn-GL. CONCLUSION: Antiulcer effects of Zn-GL were obviously found in the four experimental ulcer models, and these results contributed to its strengthening gastric mucosal defensive mechanisms, including endogenous prostaglanding E2 and gastric mucus production.