樟脑丸对雌性小鼠生殖细胞和脏器的影响

The effect of camphor ball on the germ cell and viscera of female mouse

目的 :探索由对二氯化苯制成的樟脑的生殖毒性。方法 :采用卵母细胞体外培养、体外受精等方法研究樟脑丸对小鼠卵母细胞成熟和受精能力的影响。结果 :接触樟脑丸 3 d组和 5d组对小鼠的卵巢和肝脏有明显的毒性作用 ,5d组能显著降低超排卵数 ;樟脑丸对卵母细胞生发泡破裂没有影响 ,但抑制卵母细胞第一极体的释放 ,影响其存活率及降低体外受精率。结论 :本实验结果提示 ,樟脑丸可能影响超排卵小鼠卵母细胞的成熟 ,明显降低卵母细胞的体外受精的能力 ,并表现有明显的生殖细胞毒性。

Objective: To evaluate the toxicity of camphor ball (dichlorobenzene as major component) on reproduction. Method: The maturation and the ability of in vitro fertilization (IVF) of murine oocyte were studied after being exposed to camphor ball using in vitro culture and IVF, etc. Results: The results showed that camphor ball exposure (3 days group and 5 days group) exhibited an obvious toxicity to murine ovary and liver. In the 5 days group the number of ovulation in superovulation was significantly reduced. The results also showed that camphor ball could inhibit the extruding of the first polar body and affect the quality and the viability of murine oocyte resulting in lowering the rate of IVF, but it showed little impact on germinal vesicle breakdown (GVBD). Conclusion: The results indicated that camphor ball may be hazardous to the maturation of murine oocyte, and significantly reduced the ability of IVF which may be the obvious toxicity to the reproduction of mouse.