系膜增生性肾炎中单核细胞趋化蛋白1的表达和单核巨噬细胞的浸润

Expression of monocyte chemoattractant protein-1 and infiltration of monocyte/macrophage in human mesangial proliferative glomerulonephritis

目的 观察在系膜增生性肾炎 (MsPGN)中 ,单核细胞趋化蛋白 1 (MCP 1 )的表达和单核 /巨噬细胞 (MФ)的浸润情况。方法 采用免疫组织化学和原位杂交技术观察 1 7例MsPGN患者肾活检组织。结果 ①轻度MsPGN中 ,肾小球和肾小管间质中有少量MФ浸润 ,MCP 1无明显表达 (P >0 .0 5) ;②中度MsPGN中 ,肾小球、肾小管 间质中的MФ浸润数、MCP 1表达量显著增高 (P <0 .0 1 ) ,肾小球MCP 1 +细胞数与肾小球内MФ数呈正相关 (r=0 .90 ,P <0 .0 1 ) ;MCP 1 +小管数与小管间质内MФ数亦呈正相关 (r =0 .86,P <0 .0 1 )。结论 ①MФ参与中度MsPGN中肾小球和肾间质小管的炎症病变 ;②肾小球和肾小管间质中浸润的MФ可能受MCP 1趋化作用的影响。

Objective To investigate the expression of monocyte chemoattractant protein 1(MCP 1) and infiltration of monocyte/macrophage(MФ) in renal tissue of mesangial proliferative glomerulonephritis (MsPGN). Methods MCP 1 expression and MФ infiltration in the renal biopsy tissues of 17 MsPGN patients were studied with immunohistochemical method and in situ hybridization. Results ①In mild MsPGN group, little MФ infiltration and MCP 1 expression were observed in glomerular and renal tubulointerstitial areas was found ( P >0.05). ②In medium MsPGN group, MCP 1 and CD68 expression were significantly higher than that in normal control ( P <0.01). The number of cells expressed MCP 1 in glomerular and tubulointer stitial areas showed a strong correlation with the number of MФ infiltration ( r =0.90, P <0.01, r =0.86, P < 0.01) . Conclusion ①In medium MsPGN, MФ participates in and mediates the inflammation of both glomerular and tubulointerstitial areas. ②MФ infiltration in glomerular and tubulointerstitial areas may be chemoattracted partially by MCP 1.