成年兔雪旺细胞与医用组织引导再生胶原膜联合培养后植入体内的实验研究

The Experimental Study on Culture and Transplantation of Medical Guided Tissue Regeneration Collagen Membrane Seeded with Schwann Cells

目的 :探索用组织工程方法构建人工神经的可行性。方法 :应用医用组织引导再生胶原膜作为支架与成年兔雪旺细胞培养 2周 ,以雪旺细胞胶原膜复合物的形式修复自体神经缺损。 8周后对所获得的组织工程神经移植物进行形态学研究。结果 :成年兔雪旺细胞在医用组织引导再生胶原膜上生长良好并均匀分布于支架表面 ,形成bungner带 ,且基质分泌旺盛。神经已通过移植物长入远端 ,胶原膜已吸收。结论 :雪旺细胞可以在医用组织引导再生胶原膜上得到扩增 ,形成的三维立体结构具有人工神经的基本特性 ,本研究为组织工程方法修复长段神经缺损打下基础。

Objective: To investigate the possibility of fabricating artifical nerves with tissue engineering methods. Method: The Schwann cells were cultured when they were seeded on medical guided tissue regeneration collagen membrane coating with rat tail glue and laminin together and respectively for 2 weeks. With light microscope, scanning and transmission electron microscope, the observation of the scaffolds' adsorptivity to Schwann cells , the growth and migration of the Schwann cells were made. Result: The Schwann cells could migrate on the medical guided tissue regeneration collagen membrane. Schwann cell were well distributed and formed bungner band; on which the Schwann cell produced more matrices. The regenerated nerve grew through the grafts and to the distal tip. Conclusion: Adult SC can be expanded on the medical guided tissue regeneration collagen membrane. Three dimension scaffolds with Schwann cell have the basic characteristics of the artificial nerve. These findings will give preparation for the repair of long nerve defect using tissue engineering methods.