E-钙黏蛋白及α、β-连接蛋白表达与大肠癌细胞增殖的关系

E-cadherin and α, β-catenin expression and their relationships with proliferation of colorectal adenocarcinoma cells

目的 :探讨E 钙黏蛋白 (E cad)及其相关蛋白α 连接蛋白 (α cat)、β 连接蛋白 (β cat)的表达与大肠腺癌细胞增殖的关系。方法 :用S P免疫组化方法检测 5 1例大肠腺癌中E cad、α cat、β cat的表达 ,分析其表达状况与Ki6 7标记指数 (LI)的相关性。结果 :5 1例大肠癌Ki 6 7LI为 44 7± 18 8( x±s)。 3种蛋白正常表达组与减低表达组Ki6 7LI分别为 :5 2 7± 17 5 ,38 7±17 7(E cad) ;5 0 2± 19 3 ,40 9± 17 8(α 连接蛋白 ) ;49 2± 18 2 ,42 7± 18 9(α 连接蛋白 )。E cad表达与癌细胞Ki6 7LI呈正相关 (P <0 0 5 ) ,α cat、β cat表达则与LI无关 (P >0 0 5 )。 结论 :E

Purpose To investigate the correlation between the expression of E cadherin and associated proteins, α and β catenin, and proliferation of colorectal adenocarcinoma cells. Methods E cadherin, α and β catenin immunoreactivities were evaluated in 51 patients with colorectal cancers using streptavidin peroxidase method. The results of immunostaining were compared with Ki67 labeling index ( LI ). Results Overall Ki67 LI was 44 7±18 8 ( ±s) . In groups of normal and lower protein expression, Ki67 LI was 52 7±17 5 and 38 7±17 7 for E cadherin positive cases; 50 2±19 3 and 40 9±17 8 for α cateninepithelial type cells; 49 2±18 2 and 42 7±18 9 for β catenin, respectively. E cadherin level was correlated with Ki67 LI (P <0 05), while there was no significant relationship between α or β catenin expression and Ki67 LI . Conclusion E cadherin may play a role in the regulation of the proliferation of colorectal cancer cells.