全脑缺血后大鼠纹状体边缘区原癌基因c-fos的表达及其对学习记忆功能的影响

c-fos proto-oncogene expression in the marginal division of the rat striatum following global ischemia and changes in learning and memory capacity of rats

目的观察全脑缺血时纹状体边缘区内原癌基因c-fos的表达及其对学习记忆功能的影响。方法用Pulsinelli-Brierley四血管闭塞法造成大鼠全脑缺血模型。在缺血再灌流后不同时间用免疫组化方法观察纹状体边缘区内c-fos蛋白表达的变化,同时用Y迷宫观察动物学习记忆功能的变化。结果大鼠全脑缺血后第5天,观察到纹状体边缘区内的神经元明显缺失,c-fos蛋白一过性表达增高,动物的学习记忆能力显著降低。结论全脑缺血后导致海马以及边缘区等脑区神经元损伤,可能是造成全脑缺血后学习记忆功能障碍的原因,进一步证明纹状体边缘区的功能与学习记忆密切相关。

Objective To investigatethec-fos proto-oncogeneexpressioninthemarginaldivisionof ratstriatumandchangesin thelearningandmemoryfunctionof ratsinthesettingof globalischemia.Methods Globalcerebralischemiamodelsinducedby Pulsinelli-Brierly4-vesselocclusionmethodwereused.Aftervariedtimeof reperfusion,c-fosexpressionin themarginal divisionof ratstriatumwas observedusingimmunohistochemistry,and theimpacton thelearningand memoryfunction assessedby theY-mazeaparatus.Results After5d of globalischemia,theneuronsinthemarginaldivisionof theratstriatum weredrasticallydecreasedinnumber,andtransientincreaseintheexpressionof c-fosproteinwas noted.Thelearningand memoryfunctionsof theischemicratsdeclinedas comparedwiththoseof sham-operatedrats.Conclusion Thesefindings indicatethatthe neuronalimpairmentin the hippocampusand marginaldivisionmay be responsiblefor the learningand memorydysfunctionfollowingglobalischemia,andfurtherdemonstratethatthemarginaldivisionis relatedto thelearning andmemoryfunction.