增殖细胞核抗原和p53蛋白表达与星形细胞瘤增殖调控的关系

Relations of proliferating cell nuclear antigen and p53protein expression to the regulation of astrocytoma proliferation

目的探讨星形细胞瘤中增殖细胞核抗原(PCNA)和p53蛋白表达与增殖调控的关系.方法采用免疫组化ABC法检测40例星形细胞瘤组织中PCNA和p53蛋白表达情况,分析二者与肿瘤细胞增殖调控的关系.结果PCNA的表达率:Ⅰ～Ⅱ级78.3%,Ⅲ～Ⅳ级94.1%;p53蛋白的表达率:Ⅰ～Ⅱ级43.5%,Ⅲ～Ⅳ级64.7%;PCNA表达强度与p53蛋白表达强度之间呈正相关关系.结论PCNA和p53是参与星形细胞瘤细胞增殖调控的重要基因,联合检测两者在星形细胞瘤中表达的变化可以判断肿瘤的增殖情况和恶性程度,对指导星形细胞瘤的治疗具有重要意义.

Objective To investigatetheroleof proliferatingcellnuclearantigen(PCNA)andp53proteinintheregulationof astrocytomaproliferation.Method Immunohistochemicalstaining(ABCassay)was employedto detecttheexpressionof PCNAandp53in40casesof astrocytomas.Result ThePCNAlabelingindex(PCNA-LI)was78.3%intumorsof gradeⅠtoⅡand94.1%ingradeⅢtoⅣ,showingsignificantdifferencebetweenthe2groups(P<0.05).p53-LIwasalsosignificantly higherin tumorsof gradeⅢtoⅣ(64.7%)than in tumorsof gradeⅢtoⅣ(43.5%,P<0.05).Thenuclearstainingof both PCNAandp53proteinin gradeⅢtoⅣtumorsappearedto be denserthanthatin gradeⅠtoⅡtumors,andsignificant positivecorrelationbetweentheexpressionsof these2proteinswasobservedinallthetumorsof variousgrades.Conclusion PCNAandmutantp53genesareamongtheimportantgenesthatregulatetheproliferationof theastrocytomas.Examination of theexpressionsof these2genesmayhelpassesstheproliferatingstatusandmalignancyof astorcytomas,thereforemay assistinthetreatmentof astrocytomas.