重组人粒细胞巨噬细胞集落刺激因子效价测定中最佳细胞密度的探讨

The Study of Best Cell density in titer assay of the Recombinant Granulocyte—Macrophage—Colong—Stimulating factor

重组人粒细胞巨噬细胞集落刺激因子(rh GM—CSF)效价的测定是采用 TF—1 rh GM—CSF 依赖株细胞,该细胞的增殖与 rh GM—CSF 浓度成正比,用 MTT 法测定细胞增殖程度,反映 rh GM—CSF 的活性。在测定 rh GM—CSF 的效价过程中 TF—1细胞的密度对实验结果有直接影响。

The titer assay of the recombinant Granulocyte Macrophage colong-stimulating- factor (rh GM-CSF) adopted dependant cell strain of TF-1 GM-CSF.And the multiplication rate of the cell affected the activity of GM-CSF.In the course of titer assay of GM-CSF,the cell density of TF-1 cell had direct influence on test sequence.