长穗离蠕孢引起暗色丝孢霉病一例

Phaeohyphomycosis Caused by Bipolaris Spicifera:First Case Report in China

目的分离和鉴定1株引起颅内感染的罕见病原菌。方法从患者脑脊液取材、涂片及培养,并经小鼠致病性研究重现组织病理学特征。结果根据直接镜检和培养的菌落形态及显微镜下分生孢子的大小、排列及芽管形成的位置等特征,鉴定为长穗离蠕孢,并经动物试验证实其致病性。在小鼠脑组织标本中可见菌丝及类似厚壁孢子的肿胀细胞,菌丝分隔且有色素,与暗色丝孢霉病相符。结论该病例为国内首报的由长穗离蠕孢引起的暗色丝孢霉病,其致病力强,预后不良。

Objective To isolate and identify pathogenic f ungus in a patient with intracranial infection.Methods Specimens were taken from the spinal fluid of the patient.Then,microsco py and fungal culture were done to identify the pathogen.The hi stopathologic features were reprod uced through animal pathogenicity s tudy in a mice model.Results According to the colony appearance i n culture medium,the morphological features in microscopy,such as conidia arrangement and size,germ tube forming site,this fungus was identified as Bipolaris spicifera.Hyphae and swollen hyphal cells resembling chlamydospores,septate pi gmented hyphae were observed in brain tissue specimen of mice experimental model,which were consiste nt with phaeohyphomycosis.Conclu-sion This is the first case of phaeohyphom ycosis caused by Bipolaris spicifera reported in China.