果蔗组织培养快繁技术研究

Rapid propagation techniques for tissue culture of chewing cane

采用蔗株的茎尖培养出绿苗或茎梢嫩叶诱导出愈伤组织 ,再分化出绿苗 ,均有较好效果。茎尖培养用 MS+6 - BA2 .0 m g· L- 1 +NAA0 .3m g· L- 1附加活性炭 0 .0 5 % ;MS+2 ,4 - D1.0～ 3.0 m g· L- 1对诱导愈伤组织效果较佳 ,但含 2 ,4 - D1.0 mg· L- 1的处理对黑皮果蔗仅能诱导生根而无法诱导出愈伤组织 ;MS+6 - BA1.0mg· L- 1 +KT 1.0 m g· L- 1 +NAA 0 .3mg· L- 1 对分化绿苗及其增殖效果最好 ;生根培养基选用 1/ 2 MS+IBA1.0 m g· L- 1 +NAA1.0 m g· L- 1 附加活性炭 0 .0 5 %。具有根系的完整植株的组培苗移到苗圃假植 ,待苗高 2 0 cm以上 ,分单株定植大田 ,成活率 90 %以上 ,若将丛苗再分单株袋栽成活后定植大田 ,成活率可达 10 0 %。

The rapid propagation technique for tissue culture of chewing cane was studied.The results showed that the cultivation of fascicular seedling developed from shoot tip, or the differentiation of tender seedling after inducing the callus from tender leaf at shoot tip were effective techniques for seedling growth of chewing cane.The shoot tip was cultivated on MS medium with 6\|BA 2 0 mg·L -1 , NAA 0 3 mg·L -1 and appended 0 05% active carbon The MS medium with 2,4\|D 1 0-3 0 mg·L -1 was better for callus induction, but the medium with 2,4\|D 1 0 mg·L -1 could only induce Badila chewing cane to develop roots instead of callus The MS medium with 6\|BA 1 0 mg·L -1 , KT 1 0 mg·L -1 and NAA 0 3 mg·L -1 was the best for the differentiation and reproduction of tender seedling The 1/2 MS medium with IBA 1 0 mg·L -1 , NAA 1 0 mg·L -1 and 0 05% active carbon was the best for the growth of root The survival rate of cultivation seedling with root system was over 90% by the way that seedlings were clusteredly planted at nursery first and separately planted in field after 20 cm in height.The survival rate of field planting seedling could be up to 100% when the fascicular seedlings were developed to strong sprout in bag separately.