在体细胞中实现基因打靶

Somatic gene targeting

体细胞基因打靶比较常见的ES细胞打靶是一项较新发展的技术。本文就如何设计选择打靶载体以及打靶适用的细胞系进行了较为详细的介绍。设计打靶的其他一些相关问题譬如顺序打靶、是否需要纯合DNA、高效的同源重组亦有讨论 。

Gene targeting in somatic cells is a relatively new technique compared with its pioneering gene targeting research with ES cells. However , a lot of recent advances have significantly facilitated gene targeting in somatic cells. Here in this article how to choose an efficient targeting vector and cell lines fit for research are presented in detail. And other things specifically involved in the process of gene targeting in somatic cell such as the matter of serial targeting ,need of isogenic DNA and efficient homologous recombination are also mentioned to give a overview of somatic cell gene targeting.