非小细胞肺癌6号染色体长臂的杂合性缺失的微卫星扫描分析

Genescan Analysis of the Loss of Heterozygosity on the Long Arm of Chromosome 6 in Non small Cell Lung Cancer

为探讨 6号染色体长臂上与非小细胞肺癌发生相关的微卫星位点 ,应用多重PCR对 41例非小细胞肺癌中 6号染色体长臂上的 36个微卫星位点进行扩增。PCR产物应用聚丙烯酰胺凝胶电泳分离 ,电泳结果用GeneScanTM、GenotyperTM软件进行分析。结果发现各个位点有明显不同的LOH频率 ,除D6S1 5 79在 41例非小细胞肺癌中未发现杂合性缺失外 ,其余 35个位点均有至少一个位点发生突变 ,LOH频率从 3.5 7%到 75 %不等 ,总LOH频率为 78% ( 32 /41 ) ,其中D6S30 2位点的LOH频率最高 ( 75 % )。LOH频率超过 2 0 %的位点共有 1 4个 ,主要分布在 2个区域。其中有 6个位点 :D6S45 8( 2 1 .43% )、D6S1 694( 2 6.92 % )、D6S1 71 7( 35 .71 % )、D6S1 5 65 ( 4 0 % )、D6S30 2 ( 75 % )、D6S1 70 6( 36.36% )分布在 6q2 1附近 ,具体区域是 6q1 6.3 q2 1 ;有 5个位点 :D6S1 5 5 0 ( 38.46% )、D6S2 64( 2 0 % )、D6S1 5 85 ( 2 5 % )、D6S446( 33.33% )、D6S2 81( 30 .77% )分布在 6q2 7附近 ,具体区域是 6q2 6～q2 7。因此 6q2 1、q2

To investigate if there are microsatellite loci in the long arm of chromosome 6 that have close relationship with non small cell lung cancer, Multiple PCR approach was used to analyze the 36 loci in the long arm of chromosome 6. The PCR products were analyzed in PAGE and then the electrophoresis maps were analyzed with Genescan TM and Genotyper TM . There is different LOH frequency in different loci. The total frequency of LOH in 41 lung cancers was 78%(32/41), with the highest frequency of LOH was detected on the locus D6S302 (75%). There are 14 loci which have LOH frequency more than 20% and the loci are mainly located in 2 regions: 6q16.3-q21 [6 loci [STBX] D6S458 (21.43%), D6S1694 (26.92%), D6S1717 (35.71%), D6S1565(40%),D6S302(75%),D6S1706(36.36%) and 6q26-q27(5 loci D6S1550(38.46%),D6S264(20%),D6S1585(25%),D6S446(33.33%),D6S281(30.77%)] ,There may be tumor suppressor genes located in the two regions, which have a close relationship with non small cell lung cancer. ,There may be tumor suppressor genes located in the two regions, which have a close relationship with non small cell lung cancer.