提高水稻转化效率几个主要因素的研究

The Factors of Improving Rice Transformation Efficiency

对水稻转化过程中涉及的愈伤组织的诱导、筛选、分化等进行了研究。幼胚在接种前低温预处理 4天或 7天 ,可提高愈伤组织的再生频率 ;对于继代时间较长的愈伤组织 ,高渗处理可提高抗性愈伤的比率。同时在筛选时期加大筛选压力 ,分化时去掉筛选压力 ,壮苗时加适当的选择压力 ,也可提高转化率。另外 ,在脯氨酸、二甲基亚砜对分化的影响 。

The factors influencing the rice transformation frequency have been investigated by using 8 indica and japonica rice cultivars during the transformation procedures, such as induction of calli, selection, and regeneration. The following is the results: The immature embryos were pretreated in 4℃ for 4 or 7 days before inoculation, and the regeneration frequency of calli would be increased. High osmotic treatment may increase the possibility of transformation, especially for calli of 6 months or older. The transformation frequency was improved when selection pressure was removed during differentiation period. The V-type selection may be better than the normal selection. There is a difference of differentiation ability among the calli of three periods and it is found that the calli of two or three months are the best for transformation. Proline and DMSO can decrease browning rate of calli.