葡萄球菌肠毒素A全长基因的克隆和序列测定

Cloning and sequencing of the total sequence of staphylococcal enterotoxin A gene

分析和克隆超抗原 (SAg)葡萄球菌肠毒素A(SEA)全长基因 ,为进行SAg基因应用于肿瘤导向治疗和基因治疗的研究奠定基础。设计并合成一对针对SEA全长基因的特异性引物 ,用PCR反应从产SEA的标准葡萄球菌菌株的基因组中扩增出SEA全长基因。PCR产物与克隆载体pGEM T连接后进行基因序列测定。成功地从标准葡萄球菌菌株的基因组中扩增出一条约 770bp的条带。基因序列测定表明 。

To analyze and clone the total sequence of superantigen (SAg) staphylococcal enterotoxin A (SEA) gene, and make a base for using SAg gene in tumor targeting therapy and gene therapy. A pair of primers, which were special for the total sequence of SEA gene, were designed and synthesized. The total sequence of SEA gene was enhanced from the genome of standard Staphylococci strain, which produced SEA, with PCR technique, and the gene sequence was determined after the PCR product was kinked with clone vector pGEM?T. A strip, which is about 770 bp, was enhanced from the genome of standard Staphylococci strain. The gene sequencing indicated that, it coincided totally with the published total sequence of SEA gene. The total sequence of SEA gene was cloned successfully.