TT病毒重组蛋白单克隆抗体的制备

Preparation and identification of monoclonal antibodies against recombinant protein of TT virus

采用杂交瘤技术 ,获得了 4株稳定分泌抗TTV重组蛋白的单克隆抗体杂交瘤细胞株 ,1株属IgG2bλ链、1株属IgG1κ链、2株属IgG2aκ链。 4株杂交瘤细胞培养上清液效价为 1∶80～ 1∶12 80 ,腹水效价为 1∶32万～ 1∶16

Monoclonal antibodies (McAb) against recombinant protein of TTV were produced by fusing SP2/0 myeloma cells with spleen cells from Bal b/c mice immunized with TTV recombinant proteins. Four hybridoma cell lines secreting specific antibodies against TTV recombinant proteins were obtained by screening hybridoma culture supernatants using ELISA with TTV recombinant proteins on solid phase. One of McAbs was IgG2bλ, One of McAbs was IgG1κ, Others were IgG2aκ, The titers of hybridoma culture supernatants ranged from 1∶80～1∶1 280 and from 1∶320 000～1∶1 600 000 in ascites.