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乙醛对人类神经tau磷酸化的影响 被引量：4

Effect of Acetaldehyde on Phosphorylation of Human Neuronal tau
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摘要 用乙醛对人类神经tau进行醛胺化 ,通过NCLK (neuronalcdc2 likeproteinkinase)和 [γ 3 2 P]ATP对其磷酸化 .磷酸化的产物经胃蛋白酶降解及HPLC (C 18)分析降解片段 ,发现醛胺化tau的降解物中有两个新的磷酸化肽段 (A4和A6 ) . Human neuronal tau-40 was acetylated and then phosphorylated by neuronal cdc2-like kinase (NCLK) with [gamma-P-32] ATP, and then the phosphorylated acetyl-tau (PAtau) was digested with pepsin. Compared with the phosphorylated tau (Ptau), two new fractions of radioactivity from PAtau were obtained as it was eluted by C-18 column on HPLC.
作者 华茜 聂春来 陈永辉 张岱 赫荣乔
机构地区 中国科学院生物物理研究所视觉信息加工开放实验室 北京大学第六医院
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2001年第5期761-763,共3页 Progress In Biochemistry and Biophysics
基金 国家自然科学基金 (3 9970 2 3 6) 国家重点基础研究发展规划项目 (G19990 5 40 0 7)~~
关键词 磷酸化 乙醛 老年性痴呆 人类神经tau蛋白 毒性 Tau phosphorylation acetaldehyde Alzheimer's disease
分类号 R749.16 [医药卫生—神经病学与精神病学] R996 [医药卫生—毒理学]
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共引文献86

同被引文献33

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生物化学与生物物理进展
2001年 第5期

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