对氨基苯甲酸对牙龈卟啉单胞菌胰酶样蛋白酶活性的影响

THE EFFECT OF P-AMINOBENZOIC ACID ON ACTION OF TRYPSIN-LIKE PROTEASE PRODUCED BY P.GINGIVALIS

目的 :测定不同浓度 PABA对牙龈卟啉单胞菌胰酶样蛋白酶 (TL P)活性的影响 ,以探讨血链球菌在龈下菌斑微生态平衡中的作用。方法 :在 1/ 2浓度的 BHI培养基中加入不同浓度的 PABA和一定浊度的 P.gingivalis,行常规培养。测定培养物上清液和菌细胞中 TL P活性及蛋白质含量 ,计算 TL P的比活。结果 :PABA对 P.gingivalis TL P的比活产生促进作用。结论 :PABA影响 P.gingivalis TL P的活性提示血链球菌可能对龈下菌斑的微生态平衡具调节作用。

Objectives:In order to study the effct if PABA produed by streptocccus sanguis on the microecological balance of subgingival plaque,different concentraionsof PABA was applied to see if it can influence the growth of P.gingivalis.Methods:After adding different concentrations of PABA into 1/2 concentraion of BHI media, anaerobic technique was applied to culture P.gingivalis.The products'Avalue and Vailcell(CFU/ml sample) were assayed. Results:PABA influence the growth of P.gingivalis.Conclusions:PABA that produced by Streptococcus sanguis affacting the growth of P.gingivalis isdictsthat Streptococcus sanguis has regulative effect on the microecological balance of subgingival plaque.