摘要
为研制适合于超声等影像学监测和介入治疗研究的肝癌动物模型 ,将兔后腿肌肉内的 VX2 肿瘤组织剪成 1 mm× 1 mm× 3 mm条状瘤块 ,在超声引导下将 VX2 肿瘤块接种60只新西兰白兔肝脏 ,2周后测定新西兰白兔肝肿瘤接种成功率。结果显示 ,兔肝 VX2 肿瘤接种成功 ,在超声表现为接种部位的圆形或类圆形低回声结节 ,无包膜回声 ,肿瘤有液化坏死时呈不等回声。肿瘤切面呈灰白色 ,鱼肉样 ,质硬。操作熟练后模型接种成功率 90 .5%。9只模型的自然生存期为 43 .3± 5.7d。结果提示超声引导下穿刺接种制作兔 VX2 肝癌动物模型的方法是一种操作方便简单、成功率高。
To establish VX 2 liver cancer model in rabbit, the VX 2 tissue was cut into strip shape about 1 mm×1 mm×3 mm, and were inoculated into the liver of 60 New Zealand rabbits guided under ultrasound. The successful rate of the animal model were observed 2 weeks later. Spherical or sphere-like hypoecho lesion could be found in the model. No echo of envelope could be found at the inoculated site. Heteroecho was observed when the tumor has coliquation and necrosis. The cross section of the tumor was hoariness and stiff, with fish meat-like appearance. Natural life of 9 model animals were 36～52(43.3±5.7) days. The results suggested that the ultrasound-guided percutaneous puncture-inoculation method for establishing rabbit VX 2 liver tumor model is simple and effective.
出处
《上海实验动物科学》
2001年第4期209-211,226,共4页
Shanghai Laboratory Animal Science
