具有抗多种活性氧酶功能的金属卟啉的研究——钌卟啉仿酶活性测试

Active Testing of Ruthenium Porphyrins as Mimic of Enzyme

二价金属钌卟啉 [Ru (4 - Cl- TPP) (CO ) ](1) ,[Ru (4 - Me O- TPP) (CO ) ](2 ) ,[Ru (3,4,5 - Me O-TPP) (CO) ](3)和六价金属钌卟啉 [Ru (4 - Cl- TPP) (O) 2 ](4 ) ,[Ru (4 - Me O- TPP) (O) 2 ](5 ) ,[Ru (3,4,5 - Me O-TPP) (O) 2 ](6 )被合成 .配合物 1～ 6作为超氧化物歧化酶 (SOD)和过氧化氢酶 (CAT)双功能模拟酶被测试 :核黄素 -蛋胺酸光照法证实 ,钌卟啉在不同浓度范围内有催化歧化 O.- 2 的作用 ;分光光度法证实能催化 H2 O2 分解 ;用小鼠肝匀浆法测试结果表明 :金属钌卟啉具有良好的抗脂质过氧化作用 .提出了金属钌卟啉在催化歧化超氧阴离子自由基 (O.- 2 )和催化分解过氧化氢 (H2 O2 )协同过程中的可能机理 ,并讨论了卟啉环上不同取代基对催化活性的影响 .

Three ruthenium(Ⅱ) porphyrin complexes [Ru Ⅱ(4-Cl-TPP)(CO)](1),[Ru Ⅱ(4-MeO-TPP)(CO)](2), [Ru Ⅱ(3,4,5-MeO-TPP)(CO)](3) and three ruthenium(Ⅵ) porphyrin complexes[Ru Ⅵ(4-Cl-TPP)(O) 2](4), [Ru Ⅵ(4-MeO-TPP)(O) 2 ](5),[Ru Ⅵ(3,4,5-MeO-TPP)(O) 2](6) were synthesized and their biological activities as bifunctional mimic of superoxide dismutase (SOD) and catalase (CAT) were tested. Their catalytic effects on depleting O -· 2 and decomposing H 2O 2 have been measured by riboflavin-methionine photoreduction method and spectrophotometry method respectively. The antioxidative activities of them were also studied, and the results showed these porphyrin complexes can indeed restraint the lipid peroxidation. The possible mechanism of ruthenium porphyrins catalyzing dismutation of superoxide radical and decomposition of hydrogen peroxide was put forward. In addition, the catalytic effects of different functional groups on the porphyrin rings were discussed.